Aim and background: Due to the high human-to-human transmissibility of coronavirus disease-19 (COVID-19), it has gained immense global recognition and had a devastating impact. Because of the novel nature of disease, various predisposing factors contributing to disease severity and mortality remain doubtful. This study was performed to determine the association between cycle threshold (CT) value for RT-PCR COVID-19 and severity of the disease to emphasize the role of CT value in patient management, as a lot of emphasis was being laid on reporting of CT values to the clinicians. The association between clinical symptoms and CT values was also determined in addition to its association with mortality. Methods: The study was conducted for a period of 18 months from January 2021 till June 2022. It included 212 COVID-19-positive patients who were diagnosed by RT-PCR test for COVID-19. Informed consent and history of the patients were recorded along with their clinical parameters. Data was analyzed statistically using a two-tailed t-test and the Chi-square test. Results: Although a significant association was seen between CT value and symptoms, the severity of the disease was not found to be significantly associated with CT value. Also, no statistically significant association was found between CT value and mortality due to the disease. Conclusion: Although CT value is indirectly related to the viral load, it should have no impact on patient management as the immune system shows a different response in every individual. Clinical significance: Our study adds valuable insights to this evolving landscape, emphasizing the importance of considering multiple variables in comprehensively understanding the relationship between CT values and COVID-19 severity.