Journal of South Asian Federation of Obstetrics and Gynaecology

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VOLUME 12 , ISSUE 6 ( November-December, 2020 ) > List of Articles

RESEARCH ARTICLE

Efficacy of ProEx C in Detecting Cervical Squamous Epithelial Lesions: A Cross-sectional Study

U Ashwini Nayak, Vijayshree Muthukumar, HS Sridhar, Vivaan Dutt, Madhu P Raj

Keywords : DNA topoisomerase II, Immunohistochemistry, MCM2-7 helicase, Uterine cervical neoplasms

Citation Information : Nayak UA, Muthukumar V, Sridhar H, Dutt V, Raj MP. Efficacy of ProEx C in Detecting Cervical Squamous Epithelial Lesions: A Cross-sectional Study. J South Asian Feder Obs Gynae 2020; 12 (6):383-386.

DOI: 10.5005/jp-journals-10006-1844

License: CC BY-NC 4.0

Published Online: 12-04-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objectives: To determine the diagnostic value of ProEx C in the detection of cervical squamous epithelial lesions. Materials and methods: A cross-sectional study was conducted on 60 cataloged cervical biopsy specimens. The specimens were received from incident cases for routine histopathological evaluation in MS Ramaiah Hospital. Immunohistochemical examination was performed on paraffin biopsy blocks of the specimen for nuclear and cytosolic staining with ProEx C. From the data, the proportion of positive staining cells and the average intensity of staining were assessed. Results: As per this study, the sensitivity and specificity of ProEx C were found to be 95 and 83%, respectively. Conclusion: ProEx C is an ascertained highly sensitive and specific marker. This will help to overcome the drawbacks of basic screening methods in terms of inter- and intra-observer variations and false-positive and false-negative values, and either replace or complement the less economical human papillomavirus (HPV) DNA testing in the triage for developing nations. Clinical significance: The use of ProEx C as an immunohistochemical marker is a highly reliable indicator of not only the presence of cervical squamous epithelial lesions but also the grade of the lesion. With high positive and negative predictive values for dysplasia and invasive carcinomas as well, ProEx C serves as an excellent diagnostic tool for cervical intraepithelial lesions.


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