Journal of South Asian Federation of Obstetrics and Gynaecology

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VOLUME 12 , ISSUE 6 ( November-December, 2020 ) > List of Articles


Efficacy of ProEx C in Detecting Cervical Squamous Epithelial Lesions: A Cross-sectional Study

U Ashwini Nayak, Vijayshree Muthukumar, HS Sridhar, Vivaan Dutt, Madhu P Raj

Keywords : DNA topoisomerase II, Immunohistochemistry, MCM2-7 helicase, Uterine cervical neoplasms

Citation Information : Nayak UA, Muthukumar V, Sridhar H, Dutt V, Raj MP. Efficacy of ProEx C in Detecting Cervical Squamous Epithelial Lesions: A Cross-sectional Study. J South Asian Feder Obs Gynae 2020; 12 (6):383-386.

DOI: 10.5005/jp-journals-10006-1844

License: CC BY-NC 4.0

Published Online: 12-04-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Objectives: To determine the diagnostic value of ProEx C in the detection of cervical squamous epithelial lesions. Materials and methods: A cross-sectional study was conducted on 60 cataloged cervical biopsy specimens. The specimens were received from incident cases for routine histopathological evaluation in MS Ramaiah Hospital. Immunohistochemical examination was performed on paraffin biopsy blocks of the specimen for nuclear and cytosolic staining with ProEx C. From the data, the proportion of positive staining cells and the average intensity of staining were assessed. Results: As per this study, the sensitivity and specificity of ProEx C were found to be 95 and 83%, respectively. Conclusion: ProEx C is an ascertained highly sensitive and specific marker. This will help to overcome the drawbacks of basic screening methods in terms of inter- and intra-observer variations and false-positive and false-negative values, and either replace or complement the less economical human papillomavirus (HPV) DNA testing in the triage for developing nations. Clinical significance: The use of ProEx C as an immunohistochemical marker is a highly reliable indicator of not only the presence of cervical squamous epithelial lesions but also the grade of the lesion. With high positive and negative predictive values for dysplasia and invasive carcinomas as well, ProEx C serves as an excellent diagnostic tool for cervical intraepithelial lesions.

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  1. WHO Bull. Control of cancer of the cervix, a WHO meeting. Bull World Health Organ 1986;64(4):607–618.
  2. Kurkure AP, Yeole BB. Social inequalities in cancer with special reference to South Asian countries. Asian Pac J Cancer Prev 2006;7(1):36–40.
  3. Sreedevi A, Javed R, Dinesh A. Epidemiology of cervical cancer with special focus on India. Int J Womens Health 2015;7:405–414. DOI:10.2147/IJWH.S50001.
  4. Safaeian M, Solomon D, Castle PE. Cervical cancer prevention—cervical screening: science in evolution. Obstet Gynecol Clin North Am 2007;34(4):739–760, ix. DOI:10.1016/j.ogc.2007.09.004.
  5. Guo M, Baruch AC, Silva EG, et al. Efficacy of p16 and ProExC immunostaining in the detection of high-grade cervical intraepithelial neoplasia and cervical carcinoma. Am J Clin Pathol 2011;135(2):212–220. DOI: 10.1309/AJCP1LLX8QMDXHHO.
  6. Siddiqui MT, Cohen C, Nassar A. Detecting high-grade cervical disease on ASC-H cytology: role of BD ProEx C and digene hybrid capture II HPV DNA testing. Am J Clin Pathol 2008;130(5):765–770. DOI: 10.1309/AJCPWW6V2KGXODUI.
  7. Beccati MD, Buriani C, Pedriali M, et al. Quantitative detection of molecular markers ProEx C (minichromosome maintenance protein 2 and topoisomerase IIa) and MIB-1 in liquid-based cervical squamous cell cytology. Cancer 2008;114(3):196–203. DOI: 10.1002/cncr.23496.
  8. Pinto AP, Schlecht NF, Woo TYC, et al. Biomarker (ProEx C, p16(INK4A), and MiB-1) distinction of high-grade squamous intraepithelial lesion from its mimics. Mod Pathol 2008;21(9):1067–1074. DOI: 10.1038/modpathol.2008.101.
  9. Tambouret RH. Valeur diagnostique du ProEx™ C en cytopathologie gynécologique. Ann Pathol 2008;28(1):S92–S93. DOI: 10.1016/j.annpat.2008.09.030.
  10. Badr RE, Walts AE, Chung F, et al. BD ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix. Am J Surg Pathol 2008;32(6):899–906. DOI: 10.1097/PAS.0b013e31815bbb69.
  11. Kelly D, Kincaid E, Fansler Z, et al. Detection of cervical high-grade squamous intraepithelial lesions from cytologic samples using a novel immunocytochemical assay (ProEx C). Cancer 2006;108(6):494–500. DOI: 10.1002/cncr.22288.
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