Journal of South Asian Federation of Obstetrics and Gynaecology

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VOLUME 12 , ISSUE 2 ( March-April, 2020 ) > List of Articles

LETTER TO THE EDITOR

Immunohistochemistry and Tissue Microarray Techniques to Demonstrate Human Papilloma Virus Antigen and Cell Cycle Proteins in Squamous Intraepithelial Lesions and Cancer Cervix

Rama Bajoriya, Manjiri Makde, MM Kamal

Citation Information : Bajoriya R, Makde M, Kamal M. Immunohistochemistry and Tissue Microarray Techniques to Demonstrate Human Papilloma Virus Antigen and Cell Cycle Proteins in Squamous Intraepithelial Lesions and Cancer Cervix. J South Asian Feder Obs Gynae 2020; 12 (2):116-117.

DOI: 10.5005/jp-journals-10006-1780

License: CC BY-NC 4.0

Published Online: 16-12-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Cervical cancer remains the second most common cancer among women of developing countries. Human papilloma virus (HPV) infection has been identified as leading etiological agent for cervical cancer. Cell cycle dysregulation has a central role in cervical cancer carcinogenesis. p16INK4 is a tumor-suppressor protein whose increased expression has been associated with HPV-infected dysplastic epithelium and cancer of cervix. Ki-67 is a non-histone protein expressed in nucleus during whole cell cycle except G0 and G1. It constitutes an efficient marker of proliferating cells. It has its utility in distinguishing non-diagnostic atypia from squamous intraepithelial lesion (SIL). Our aim was to evaluate the usefulness of p16INK4 and Ki-67 in the diagnosis of spectrum of SIL cervical carcinoma, to know the genotype of HPV positivity in invasive cervical cancers using the TMA technique and to demonstrate the importance of the technique of tissue microarray (TMA) as an educational activity. Paraffin blocks, histology slides, and clinical data of 354 cervical biopsies sent to department's histology laboratory at GMC Nagpur were retrieved. The lesions were classified as LSIL, HSIL, and cervical carcinoma as per WHO guidelines. All cases of adenocarcinoma and randomly selected cases of squamous cell carcinoma were subjected to p16INK4 and Ki-67 labeling using TMA technique. Ki-67 and p16INK4 are aberrantly expressed in LSIL–HSIL–carcinoma sequence. p16 has significant association with severity of histological lesions. Also, HPV positivity with 16 and 18 was 100% in all the carcinomas.


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