Journal of South Asian Federation of Obstetrics and Gynaecology

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VOLUME 16 , ISSUE 2 ( March-April, 2024 ) > List of Articles


Ultraviolet Spectroscopy vs Congo Red Card Test—A Step Forward toward Early Prediction of Preeclampsia: A Case–Control Study

Neena Gupta, Garima Gupta, Uruj Jahan, Sonali Bisht

Keywords : Adverse maternal outcome, Congophilia, Congo red paper test, Misfolded proteins, Preeclampsia, Prevention, Spectroscopy

Citation Information : Gupta N, Gupta G, Jahan U, Bisht S. Ultraviolet Spectroscopy vs Congo Red Card Test—A Step Forward toward Early Prediction of Preeclampsia: A Case–Control Study. J South Asian Feder Obs Gynae 2024; 16 (2):192-197.

DOI: 10.5005/jp-journals-10006-2325

License: CC BY-NC 4.0

Published Online: 23-02-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: Comparative evaluation of ultraviolet–visible (UV–Vis) spectroscopy and Congo red card-based test in the diagnosis of preeclampsia. Materials and methods: In this case–control study, UV–Vis spectroscopy and Congo red card test were done using mid-stream clean catch urine samples collected from 98 cases and 98 controls. The results obtained were used to calculate the specificity and sensitivity of each test. Results: Out of 98 cases, 76 showed increased absorbance in UV–Vis spectroscopy while 69 showed Congo red positivity. Thus, the ultraviolet (UV)-absorbance method was found to be more sensitive (77.53) and specific (80.61) compared to the Congo red paper-based test (sensitivity, 70.40; specificity, 64.28) in the detection of the misfolded proteins present in the urine samples of the preeclamptic patients. Conclusion: The UV–Vis absorption spectroscopy is a renowned yet innovative technique in the diagnosis of preeclampsia. It is more sensitive and specific to the existing Congo red paper-based test. Clinical significance: Several studies show that urine of preeclampsia patients shows the presence of misfolded proteins which are present about 2 weeks before the onset of symptoms, that is, clinical diagnosis. This creates scope for early detection and diagnosis of preeclampsia. In this study, we establish the role of spectroscopy as a novel test in diagnosing misfolded proteins in preeclamptic patients and compare its diagnostic performance to the highly acclaimed Congo red card test.

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