Aims and objectives: To study the prevalence of vaginal infections in pregnant women and its implications in pregnancy. Study design: Prospective case control study. Setting: Obstetric unit of tertiary care hospital. Materials and methods: Overall 200 cases were enrolled, 100 symptomatic and 100 asymptomatic women. A thorough general physical examination and P/S examination were carried out and the vaginal mucosa was inspected for the presence of erythema, lesions, and discharge. High vaginal swabs were then collected from the posterior fornix with cotton-swab, avoiding contamination with the cervical mucus. Subjected to KOH test followed by wet mount preparation and Gram's staining, each second high-vaginal swab was then cultured using a Sabouraud's Dextrose agar plate for Candida spp., MacConkey's agar, and Chocolate agar in aerobic conditions, respectively, for gram-negative bacilli and chocolate agar for the isolation of group B streptococci. Identification of the aerobic culture isolates was performed using 41 standard biochemical tests. Afterward, colonies grown on SDA agar were subjected to growth on CHROM agar for speciation of Candida. Diagnosis of BV was made using Amsel's criteria and Gram's stain Nugent's scoring system. Results: The prevalence of vaginal infections in the present study was 22.5%. Incidence of BV was found to be 9%. Incidence of candidiasis was found to be 13.5%. The analysis in the present study also showed that there were greater odds of vaginal infections among women with clinical symptoms (37%) as compared to asymptomatic women (8%). Conclusion: In conclusion, vaginal infections are highly prevalent among pregnant women and a frequent cause of adverse obstetric outcome and their clinical diagnosis should be a two-stepped approach combining both clinical and microbiological diagnostic methods.