Journal of South Asian Federation of Obstetrics and Gynaecology

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VOLUME 11 , ISSUE 4 ( July-August, 2019 ) > List of Articles

Original Article

Evaluation of Diagnostic Value of Pouch of Douglas Fluid in Comparison to Endometrial Biopsy Samples for the Diagnosis of Genital Tuberculosis by Real-time Polymerase Chain Reaction

Parul Sinha, Bharti Malhotra, Romika Dawra, Shivra Batra, Dinesh K Jain

Keywords : Endometrial biopsy, Genital tuberculosis, POD fluid

Citation Information : Sinha P, Malhotra B, Dawra R, Batra S, Jain DK. Evaluation of Diagnostic Value of Pouch of Douglas Fluid in Comparison to Endometrial Biopsy Samples for the Diagnosis of Genital Tuberculosis by Real-time Polymerase Chain Reaction. J South Asian Feder Obs Gynae 2019; 11 (4):255-257.

DOI: 10.5005/jp-journals-10006-1708

License: CC BY-NC 4.0

Published Online: 01-08-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Objective: The aim of this study was to evaluate the benefits of using two types of samples: the endometrial biopsy (EB) and the pouch of Douglas (POD) fluid by real-time polymerase chain reaction (PCR) for an early and accurate diagnosis of female genital tuberculosis (FGTB). Materials and methods: Two samples, one EB and second POD fluid, from each patient (total 173 patients) were subjected to IS6110-based real-time PCR assay for the diagnosis of FGTB. Results: Among 173 patient samples received, a total of 32.94% (57/173) patients were positive for MTB by either or both sample types. Endometrial biopsy samples were positive in 19.60% (34/173) of all EB samples for MTB, and POD fluid samples were positive in 24.27% (42/173) of all POD fluid samples for MTB. Among the total MTB-positive patients, 26.31% (15/57) patients had only EB sample as positive, whereas in 40.35% (23/57) patients only POD samples were positive. Conclusion: When genital tuberculosis is suspected clinically, it is better to opt for performing less invasive procedure like testing of POD fluid for MTB, and if it is negative, then further invasive procedure to obtain EB may be chosen for diagnosis.

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